There are two main types of fluorescent antibodies used in histology: 1. Direct Fluorescent Antibodies: These antibodies are directly conjugated with a fluorescent dye. They bind directly to the target antigen, providing a straightforward and quick method of detection. 2. Indirect Fluorescent Antibodies: In this method, primary antibodies bind to the target antigen, and then secondary antibodies, which are conjugated with fluorescent dyes, bind to the primary antibodies. This approach can amplify the signal and increase sensitivity.
