To achieve optimal results with Fast Red staining, consider the following tips:
Optimize Incubation Times: Adjust incubation times for primary and secondary antibodies to ensure adequate binding and signal development. Control Enzyme Activity: Ensure that the enzyme activity is well-preserved during tissue preparation and staining to achieve consistent results. Use Appropriate Counterstains: Choose counterstains that provide a good contrast with Fast Red, such as hematoxylin, to enhance visualization.
