In immunohistochemistry, Fast Red is often combined with alkaline phosphatase-conjugated secondary antibodies. The procedure typically involves the following steps:
Preparation of tissue sections. Blocking of non-specific binding sites. Application of primary antibodies specific to the target antigen. Application of secondary antibodies conjugated to alkaline phosphatase. Incubation with Fast Red substrate, resulting in a red precipitate at the site of the antigen.
