Fast Red is widely used in various applications, including:
Immunohistochemistry: Fast Red is used as a chromogen in IHC to detect the presence of specific antigens or proteins in tissue sections. It provides a vivid red color that contrasts well with other counterstains. Enzyme Histochemistry: This method uses Fast Red to localize enzymes such as alkaline phosphatase in tissue sections. The reaction between the enzyme and the substrate results in a red precipitate. In Situ Hybridization: Fast Red can be used to detect RNA or DNA sequences in tissue samples, providing a strong visual contrast.