Controlling variables is essential for reproducibility and consistency. Standardize variables such as:
Reagent Quality: Use high-quality reagents and prepare fresh solutions to avoid degradation and contamination. Incubation Times: Adhere to recommended incubation times for each reagent to prevent overstaining or understaining. Temperature: Maintain consistent temperatures during staining and washing steps to ensure uniform results. pH Levels: Monitor and adjust the pH of staining solutions to optimize binding interactions between stains and tissue components.
