The choice of blocking buffer depends on several factors, including the type of tissue, the primary and secondary antibodies used, and the detection method. It's often beneficial to test different blocking buffers to determine which one provides the best results for a specific application. Some tips include:
- Compatibility: Ensure the blocking buffer is compatible with the antibodies and detection reagents. - Background Reduction: Choose a buffer that effectively reduces background staining without affecting specific binding. - Ease of Use: Some commercial blocking buffers are more convenient and consistent than homemade solutions.
