Detection of Zn SOD in histological samples can be achieved through various techniques: - Immunohistochemistry (IHC): This technique uses specific antibodies to detect Zn SOD in tissue sections. The antibodies bind to Zn SOD, and the binding is visualized using chromogenic or fluorescent labels. - Western Blotting: Although not a direct histological technique, Western blotting can be used to confirm the presence of Zn SOD in extracted proteins from tissues. - Enzyme Activity Assays: These assays measure the enzymatic activity of Zn SOD in tissue extracts, providing functional evidence of its presence.
