What are the Common Challenges in Studying Zn SOD?
Studying Zn SOD presents several challenges: - Antibody Specificity: Ensuring that antibodies used in IHC or Western blotting are specific to Zn SOD and do not cross-react with other proteins. - Activity Assays: Accurately measuring Zn SOD activity can be complicated due to the presence of other superoxide dismutases in the tissue. - Tissue Preservation: Proper tissue fixation and preservation are crucial to maintain the integrity of Zn SOD for reliable detection and analysis.