In histological studies, vascularization can be examined using various staining techniques. Commonly used stains include Hematoxylin and Eosin (H&E) which provide a general overview, and specific markers like CD31 or von Willebrand factor (vWF) that highlight endothelial cells. Advanced techniques such as immunohistochemistry and in situ hybridization can also be employed to study the expression of specific genes and proteins involved in vascularization.