The assessment of clear margins involves several steps: 1. Tissue Collection: The tissue sample is collected during surgery. 2. Fixation and Sectioning: The tissue is then fixed, usually with formalin, and cut into thin sections. 3. Staining: The sections are stained using techniques like Hematoxylin and Eosin (H&E) staining. 4. Microscopic Examination: A histopathologist examines the stained sections under a microscope to check for the presence of cancer cells at the edges.
