The specificity of IHC markers is ensured by using highly specific antibodies that bind only to the target antigen. Additionally, controls are used to validate the specificity of the staining. These controls include:
- Positive Control: Tissues known to express the target antigen. - Negative Control: Tissues known not to express the target antigen or sections stained without primary antibodies. - Isotype Control: Using an irrelevant antibody of the same isotype to account for non-specific binding.
