Histological examination of stromal tissue involves several techniques:
Light Microscopy: Stained tissue sections are observed under a microscope to identify the cellular and extracellular components. Electron Microscopy: Provides high-resolution images of the ultrastructure of stromal components, such as the arrangement of collagen fibers. Immunohistochemistry (IHC): Uses antibodies to detect specific proteins, allowing for the localization of different cell types and ECM components. Histochemical Staining: Techniques like Masson's Trichrome and Van Gieson's stain are used to differentiate between various tissue elements such as collagen and muscle fibers.
