Several methods are employed to ensure chemical stability in histology:
Fixation:Fixatives such as formaldehyde and glutaraldehyde are used to preserve tissue samples by cross-linking proteins and preventing enzymatic degradation. Embedding: Embedding tissues in paraffin wax or resin helps to stabilize them and maintain their structure during sectioning and staining. Proper Storage: Storing reagents and samples at appropriate temperatures and conditions (e.g., refrigeration, airtight containers) helps to maintain their chemical stability. pH Control: Maintaining the correct pH in staining solutions and buffers is essential, as fluctuations can lead to chemical instability and inconsistent results.
