Single cell sequencing typically involves several steps: - Isolation of individual cells using techniques like fluorescence-activated cell sorting (FACS) or microfluidics. - Amplification of the genetic material, since the DNA or RNA from a single cell is insufficient for sequencing. - Library Preparation where the genetic material is prepared for sequencing. - Sequencing using high-throughput technologies such as next-generation sequencing (NGS). - Data Analysis to interpret the vast amount of data generated.
