In histology, SHM can be studied using various techniques such as immunohistochemistry, in situ hybridization, and flow cytometry. These techniques allow researchers to visualize and quantify the expression of key molecules involved in SHM, such as AID, and to identify the localization of B cells within the germinal centers. Additionally, next-generation sequencing can be used to analyze the mutations introduced in the Ig genes at a molecular level.
