In histological studies, SASP can be identified through:
1. Immunohistochemistry (IHC): This technique uses antibodies to detect specific SASP components in tissue sections. Common markers include IL-6, IL-8, and MMPs. 2. In situ hybridization: This method detects mRNA expression levels of SASP factors in tissue samples. 3. Histochemical staining: Techniques such as β-galactosidase staining (senescence-associated β-gal) are used to identify senescent cells.
