Why is End to End Ligation Important in Histology?

In histology, end to end ligation is pivotal for several reasons:

Molecular Cloning: It enables the insertion of specific DNA fragments into vectors, which can then be introduced into host cells for further study.
Gene Expression Studies: By ligating specific sequences, researchers can create constructs to study the expression of particular genes in different tissues.
Tissue Engineering: Ligation techniques are employed to modify genetic material for the development of tissues and organs in regenerative medicine.
Diagnostic Applications: It aids in the development of probes and primers essential for detecting genetic mutations and diseases.