Rigidity in tissues can be measured using several techniques. One common method is atomic force microscopy (AFM), which measures the mechanical properties at the nanoscale by applying a controlled force to the tissue and recording its response. Another method is magnetic resonance elastography (MRE), a non-invasive imaging technique that measures the stiffness of tissues by visualizing the propagation of mechanical waves. Additionally, optical tweezers and microindentation techniques are also used to assess tissue rigidity.