Orientation is achieved through meticulous handling during the tissue processing stages:
Fixation: Tissues are preserved in a fixative to maintain their structure and prevent decomposition. Embedding: Tissues are embedded in a medium, usually paraffin wax, to support them during sectioning. Sectioning: Thin sections of the tissue are cut using a microtome. The orientation of the tissue block in the microtome is critical for obtaining meaningful sections. Mounting: Sections are placed on slides in a specific orientation to ensure that the desired view is achieved under the microscope.
