How Is Mechanical Irritation Studied in Histology?

Histologists employ various techniques to study the effects of mechanical irritation. Tissue samples are often collected and stained using specific dyes like Hematoxylin and Eosin (H&E) to highlight cellular and extracellular components. Immunohistochemistry can be used to identify specific proteins involved in the stress response, such as heat shock proteins and inflammatory markers. Advanced imaging techniques like electron microscopy may also be utilized to observe ultrastructural changes.