Isolation of MSCs commonly involves harvesting bone marrow aspirates or other tissues, followed by density-gradient centrifugation to separate mononuclear cells. These cells are then cultured in plastic-adherent conditions using specific growth media. Over time, MSCs proliferate and form colonies known as colony-forming unit fibroblasts (CFU-Fs). Subculturing and passaging techniques are employed to expand the MSC population for further research or therapeutic applications.
