How is Irreversible Injury Identified Histologically?
Irreversible injury can be identified using various histological techniques. Common methods include:
- Light Microscopy: Observing morphological changes in stained tissue sections, such as nuclear alterations and cytoplasmic eosinophilia. - Electron Microscopy: Providing detailed images of organelle damage, especially mitochondrial swelling and cristae disruption. - Immunohistochemistry: Detecting specific markers of cell death, such as cleaved caspase-3 for apoptosis or necrotic markers.
