fish (fluorescence in situ hybridization)

How Does FISH Work?

The fundamental process of FISH involves several steps:
1. Sample Preparation: Biological samples, such as tissue sections or cells, are fixed onto a slide.
2. Probe Labeling: Single-stranded DNA or RNA probes are labeled with fluorescent dyes.
3. Hybridization: The labeled probes are applied to the sample, where they hybridize to their complementary sequences.
4. Washing: Excess probes are washed away to reduce background noise.
5. Detection: The sample is examined under a fluorescence microscope to visualize the hybridized probes.

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