The process of histological subtyping involves several steps:
Tissue Collection: A biopsy or surgical resection is performed to collect a tissue sample. Fixation and Processing: The sample is preserved using fixatives like formalin, embedded in paraffin, and sectioned into thin slices. Staining: Sections are stained with dyes such as Hematoxylin and Eosin (H&E) to highlight cellular structures. Microscopic Examination: Pathologists examine the stained sections under a microscope to identify cellular patterns and structures indicative of specific subtypes.
