What are the Steps Involved in Staining with Resorcin Fuchsin?
The process of staining with resorcin fuchsin typically involves several steps:
Fixation: Tissue samples are fixed to preserve their structure. Dehydration: The samples are dehydrated using alcohol to prepare them for staining. Oxidation: The resorcin fuchsin solution is applied, allowing oxidation to occur. Staining: The elastic fibers within the tissue take up the stain. Differentiation: Excess stain is removed to enhance contrast. Mounting: The stained tissue is mounted on slides for microscopic examination.
