In histology, the study of Hedgehog signaling involves various techniques to visualize and analyze the expression and localization of Hedgehog ligands and their receptors. Immunohistochemistry (IHC) and in situ hybridization (ISH) are commonly used to detect Hedgehog proteins and mRNA in tissue sections. Additionally, genetic models and organoid cultures are employed to dissect the functional roles of Hedgehog signaling in tissue development and disease.
