In histological studies, GATA4 expression and localization are typically assessed using techniques such as:
Immunohistochemistry (IHC): This method uses antibodies specific to GATA4 to detect its presence in tissue sections, providing information on its spatial distribution. In situ hybridization: This technique allows for the detection of GATA4 mRNA within tissue samples, offering insights into gene expression patterns. Western Blotting: Used to quantify GATA4 protein levels in tissue extracts, complementing histological analyses.
