In histological applications, tissue samples are often prepared and subjected to Fehling's test to identify the presence of reducing sugars. The procedure generally involves:
Fixing and preparing the tissue sample. Sectioning the sample into thin slices. Applying Fehling's solution to the tissue sections. Observing any color changes under a microscope.
A positive result is indicated by the formation of a reddish-brown precipitate of copper(I) oxide, suggesting the presence of reducing sugars within the tissue.
