One of the main challenges in studying Caspase 7 is distinguishing between its inactive and active forms. Since Caspase 7 exists in an inactive proenzyme form in healthy cells, only the active form is relevant to apoptosis. Additionally, the transient nature of apoptosis means that detecting active Caspase 7 requires precise timing and sensitive detection methods. Another challenge is the potential cross-reactivity of antibodies used in IHC, which can lead to non-specific staining and misinterpretation of results.
