The application of coating materials varies depending on the type of material and the intended use. Here are some common methods:
Embedding: Tissues are infiltrated with paraffin wax or resin to provide support during sectioning. The material is melted, and the tissue is submerged, then allowed to cool and solidify. Surface Coating: Thin layers of metals or other materials are applied to the surface of specimens, often using techniques like sputter coating or vapor deposition. Slide Coating: Slides are treated with substances like gelatin or silane to improve tissue section adhesion. The coating is applied as a liquid and allowed to dry before use.