The procedure for Perls' staining involves the following steps:
Fix the tissue in an appropriate fixative, such as formalin. Embed the tissue in paraffin and cut into thin sections. Deparaffinize and rehydrate the tissue sections. Incubate the sections in a mixture of hydrochloric acid and potassium ferrocyanide solution. Wash the sections thoroughly with distilled water. Counterstain with a nuclear stain, such as neutral red or nuclear fast red. Dehydrate, clear, and mount the sections for microscopic examination.
