What is the Protocol for Using Biebrich Scarlet Acid Fuchsin?

The staining protocol involves several steps. First, tissue sections are fixed and then treated with a mordant, such as Bouin's solution, to enhance staining. After this, the sections are stained with Biebrich Scarlet, which colors the cytoplasm and muscle fibers. Following this, the sections are treated with a phosphomolybdic-phosphotungstic acid solution to differentiate the muscle fibers from collagen. Finally, the sections are stained with Acid Fuchsin, which highlights the collagen fibers.