The celloidin embedding process involves several steps to ensure the tissue is adequately prepared for sectioning:
Dehydration: Tissues are dehydrated through a series of graded alcohols to remove water, which can interfere with celloidin infiltration. Infiltration: The tissue is then infiltrated with celloidin, typically dissolved in a mixture of alcohol and ether, over several days to ensure thorough penetration. Hardening: The infiltrated tissue is hardened by exposing it to chloroform vapors or by immersing it in a chloroform-alcohol solution. Sectioning: Once hardened, the tissue block can be sectioned using a microtome equipped for celloidin-embedded specimens.
