Histological studies often employ various techniques to study the Nrf2 pathway. Immunohistochemistry (IHC) is commonly used to detect Nrf2 and its target proteins in tissue samples. Additionally, techniques such as Western blotting and quantitative PCR (qPCR) are used to measure the expression levels of Nrf2 and its downstream targets. These studies provide insights into the spatial and temporal activation of the Nrf2 pathway in different tissues.
