Several factors contribute to cell line variability, including:
Genetic drift: Random mutations that accumulate over time can lead to genetic changes within the cell line. Epigenetic modifications: Changes in DNA methylation and histone modification can alter gene expression without changing the DNA sequence. Culture conditions: Variations in media composition, temperature, and other environmental factors can affect cell growth and behavior. Passage number: Repeated subculturing of cells can lead to changes in their characteristics and introduce variability. Contamination: Introduction of microbial agents or cross-contamination with other cell lines can alter the properties of the cell line.
