Stereology employs mathematical and statistical methods to extrapolate three-dimensional information from two-dimensional microscopic images. Common techniques include:
Point Counting: Estimating volume by overlaying a grid of points on the tissue section and counting the number of points that fall on the structure of interest. Optical Disector: A method for counting the number of particles (e.g., cells) in a three-dimensional volume by analyzing a series of optical sections. Cavalieri Principle: Estimating the volume of an object by summing the areas of its cross-sections multiplied by the distance between them.
