What are the Steps Involved in Using the Cavalieri Principle?
1. Section the Tissue: The first step is to cut the tissue into a series of parallel, equally spaced sections. The distance between sections should be consistent. 2. Measure the Areas: Each section’s area is then measured, typically using image analysis software. 3. Calculate the Volume: The volume is calculated by summing the areas of the sections and multiplying by the distance between the sections.