The choice of embedding medium, such as paraffin or resin, significantly impacts tissue quality. Proper embedding ensures that tissues are well-supported during sectioning and staining. Key considerations include:
Compatibility with Tissue Type: Different tissues may require specific embedding media for optimal results. Complete Infiltration: Ensure the embedding medium fully infiltrates the tissue to avoid air pockets and incomplete sections. Proper Orientation: Correct orientation of tissues during embedding is essential for obtaining accurate sections.