How is Cytogenetic Analysis Performed on Tissue Samples?
To perform cytogenetic analysis on tissue samples, a series of steps are followed:
Tissue Collection: Samples are collected through biopsy or surgical excision. Cell Culture: Cells from the tissue sample are cultured to obtain a sufficient number for analysis. Chromosome Preparation: Cells are arrested in metaphase, a stage where chromosomes are most visible, and then stained or probed using specific techniques. Microscopic Examination: Chromosomes are examined under a microscope or using imaging systems to detect abnormalities. Data Interpretation: The results are analyzed to understand the genetic alterations present in the tissue sample.
