Preventing tissue damage involves meticulous attention to each step of histological processing. Some key preventive measures include:
Optimizing Fixation Protocols: Use appropriate fixatives and ensure proper fixation time to preserve tissue morphology. Ensuring Adequate Dehydration: Follow standardized dehydration protocols to remove all water content before embedding. Proper Embedding Techniques: Ensure correct orientation and complete infiltration of embedding medium, such as paraffin. Regular Maintenance of Equipment: Keep microtome blades sharp and ensure they are properly aligned to avoid mechanical damage. Standardizing Staining Procedures: Use consistent staining protocols and quality control measures to achieve reproducible results.
