The preparation of tissue cultures involves several steps:
Isolation: Cells or tissues are obtained from their original environment, often using enzymatic digestion or mechanical methods. Medium Preparation: A suitable culture medium is prepared, containing essential nutrients, growth factors, and hormones. Seeding: Cells are seeded into culture dishes or flasks and incubated under controlled conditions (temperature, humidity, CO2 concentration). Maintenance: Regular monitoring and maintenance, including medium replacement and passaging of cells to avoid overconfluence.
