The method of introducing tracers depends on the type of study. Common methods include:
1. Injection: Direct injection into tissues or the bloodstream. 2. Perfusion: Circulating the tracer through the blood vessels of an organ. 3. Incubation: Incubating tissue sections with tracers in vitro. 4. Micropipette Delivery: Using micropipettes to deliver tracers at specific sites within tissues.
