Proper preparation of tissue samples is crucial for accurate identification. Steps include:
Fixation: Use a fixative like formalin to preserve tissue morphology and prevent degradation of mucopolysaccharides. Embedding: Embed the tissue in paraffin or resin to provide support for thin sectioning. Sectioning: Cut the embedded tissue into thin sections (3-5 microns) using a microtome. Staining: Apply the chosen staining technique to visualize mucopolysaccharides under a microscope.
