In histology, t-SNAREs are studied using a combination of techniques such as immunohistochemistry, electron microscopy, and fluorescence microscopy. Immunohistochemistry involves the use of specific antibodies to label t-SNAREs in tissue sections, allowing for their localization and quantification. Electron microscopy provides detailed images of the ultrastructure of cells, revealing the precise arrangement of SNARE complexes. Fluorescence microscopy, often coupled with live-cell imaging, enables the dynamic observation of vesicle fusion events in real-time.
