Using non-isotonic solutions can lead to significant issues:
1. Hypertonic Solutions: These solutions have a higher osmotic pressure than the cytoplasm, causing cells to lose water and shrink (crenate). This can distort cell morphology and affect histological analysis. 2. Hypotonic Solutions: These solutions have a lower osmotic pressure, leading to water influx into cells, causing them to swell and potentially burst. This can also distort tissue structure and lead to inaccurate results.
