To optimize fixative volume, consider the following steps:
Sample Preparation: Trim tissues to an appropriate size to facilitate better penetration. Ensure Adequate Volume: Always use a volume at least 10 times the tissue size, adjusting based on the fixative type and tissue characteristics. Monitor Fixation Time: Allow sufficient time for fixation, which can vary from a few hours to several days depending on the tissue and fixative. Avoid Fixative Dilution: Ensure the fixative is at the correct concentration to avoid incomplete fixation.
