The application of special stains generally involves a series of steps including fixation, embedding, sectioning, and staining. The process varies depending on the type of stain being used:
Fixation: Tissue samples are preserved using fixatives like formalin to prevent decomposition. Embedding: Fixed tissues are embedded in paraffin or other media to provide support during sectioning. Sectioning: Thin tissue sections are cut using a microtome. Staining: Specific dyes and chemicals are applied to the tissue sections to achieve the desired staining effect.
