HEK293 cells are cultured in a controlled environment using cell culture techniques. They are typically grown in a medium such as Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS). The cells are maintained in a humidified incubator with 5% CO2 at 37°C. Regular subculturing is necessary to keep the cells healthy and to prevent over-confluence.
