The process of tissue culture involves several steps:
Preparation: Tissues are sterilized to prevent contamination, followed by their dissection into smaller pieces. Medium Selection: The tissues are placed in a nutrient-rich medium that supports cell growth. The choice of medium depends on the type of tissue and the purpose of the culture. Incubation: The cultures are incubated under controlled conditions (temperature, humidity, CO2 levels) to facilitate cell growth and division. Observation and Maintenance: Regular monitoring is essential to ensure optimal growth and to detect any contamination. The medium is periodically changed to supply fresh nutrients.
