Bead mills operate by agitating beads within a sample tube. When the tube is subjected to vigorous motion, the beads collide with the sample, breaking it down into smaller particles. The efficiency of this process hinges on factors such as bead size, bead material, and the type of motion applied. Typically, bead mills use beads made of materials like glass, zirconia, or stainless steel, chosen based on the hardness and brittleness of the sample.